Pilot project on Xylella fastidiosa to reduce risk assessment uncertainties
نویسندگان
چکیده
منابع مشابه
EXPLOITING XYLELLA FASTIDIOSA PROTEINS FOR PIERCE’S DISEASE CONTROL Project Leaders:
The principal objective of this project is to construct and express in test plants, and then in grapevine rootstock, a protein or protein chimera capable of inactivating Xylella fastidiosa (Xf), the causative agent of Pierce’s disease (PD) of grapevine. Prior results from this project identified MopB as a, or possibly the, major outer membrane protein of Xf. We have shown that MopB is accessibl...
متن کاملROLES OF XYLELLA FASTIDIOSA PROTEINS IN VIRULENCE Project Leader:
This work derives from a preliminary experiment by Civerolo and Bruening in which Chenopodium quinoa (Cq) was found to develop a localized chlorosis 24-48 hr after leaves were infiltrated with a suspension of live or heat-killed Xylella fastidiosa (Xf) cells. Excised electrophoresis gel regions were assayed for chlorosis-inducing activity, which was associated with a protein band with mobility ...
متن کاملANALYSIS OF XYLELLA FASTIDIOSA TRANSPOSON MUTANTS AND DEVELOPMENT OF AN EN PLANTA XYLELLA FASTIDIOSA PLASMID VECTOR Project Leader: Cooperator:
We have developed an autonomously replicating Xylella fastidiosa (Xf)/E. coli plasmid that efficiently transforms Xf; unfortunately this plasmid was not stably maintained in Xf cells en planta or without antibiotic selection in vitro. Another plasmid, containing an Xf native plasmid, a Kan cassette and cloned in pUC18 was also constructed and shown to be unstable without antibiotic selection. 1...
متن کاملProject Risk Assessment Framework
This study presents a framework for calculating the risk of various projects, especially projects under uncertain circumstances. First, the related literature is reviewed and then the relationship between risk and projects is examined. Using a case study an approach is provided to determine the project risk in uncertain circumstances where sufficient data is not available for decision-making. I...
متن کاملINHIBITION OF XYLELLA FASTIDIOSA POLYGALACTURONASE TO PRODUCE PIERCE’S DISEASE RESISTANT GRAPEVINES Project Leader:
Polygalacturonases (PG) (EC 3.2.1.15), catalyze the random hydrolysis of 1, 4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. Xylella fastidiosa (Xf) possesses a single PG gene, pglA (PD1485) and Xf mutants deficient in the production of PG result in lost pathogenicity and a compromised ability to systemically infect grapevines. We have cloned the pglA gene into a number o...
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ژورنال
عنوان ژورنال: EFSA Supporting Publications
سال: 2016
ISSN: 2397-8325,2397-8325
DOI: 10.2903/sp.efsa.2016.en-1013